Plasmid_Backbone

Part:BBa_J72204:Design

Designed by: Josh Kittleson   Group: Anderson Lab   (2012-09-17)


ampicillin resistant split R6K vector w/ O16 HAs and FRT-Kan-FRT


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3940
    Illegal XbaI site found at 45
    Illegal XbaI site found at 1114
    Illegal PstI site found at 16
    Illegal PstI site found at 1652
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3940
    Illegal PstI site found at 16
    Illegal PstI site found at 1652
    Illegal NotI site found at 1756
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Illegal EcoRI site found at 3940
    Illegal BglII site found at 3949
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3940
    Illegal XbaI site found at 45
    Illegal XbaI site found at 1114
    Illegal PstI site found at 16
    Illegal PstI site found at 1652
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3940
    Illegal XbaI site found at 45
    Illegal XbaI site found at 1114
    Illegal PstI site found at 16
    Illegal PstI site found at 1652
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2402


Design Notes

n/a


Source

n/a

References